. 2022 Aug 25;13:109. doi: 10.1186/s40104-022-00747-7

Table 5.

The free amino acid composition (mg/100 g tissue) and IMP content (nmol/L) in the muscle of grass carp

Items	Dietary protein levels						Linear		Quadratic
Items	15.91%	19.39%	22.10%	25.59%	28.53%	31.42%	F	P	F	P
Aspartate	0.20 ± 0.17^a	0.74 ± 0.72^ab	0.97 ± 0.43^b	0.92 ± 0.30^b	0.58 ± 0.28^ab	0.51 ± 0.46^ab	0.50	0.49	10.84	< 0.01
Glutamate	6.41 ± 1.93^a	9.69 ± 2.93^b	6.79 ± 1.96^ab	7.53 ± 1.69^ab	7.39 ± 3.90^ab	8.38 ± 1.15^ab	0.20	0.66	0.00	0.97
Glutamine	56.51 ± 14.78^a	63.74 ± 10.21^ab	73.04 ± 4.20^b	65.89 ± 6.25^ab	63.39 ± 17.31^ab	65.94 ± 12.29^ab	0.94	0.34	2.58	0.12
Glycine	189.08 ± 31.76^b	196.48 ± 44.21^b	131.06 ± 26.76^a	103.88 ± 17.22^a	101.95 ± 22.60^a	97.33 ± 12.35^a	65.65	< 0.01	3.47	0.07
Alanine	73.95 ± 7.62^d	56.37 ± 7.05^c	40.97 ± 9.41^b	42.00 ± 8.77^b	37.98 ± 7.82^ab	29.00 ± 8.03^a	100.33	< 0.01	8.42	< 0.01
Proline	253.51 ± 54.40^a	296.52 ± 64.87^ab	313.76 ± 48.70^ab	336.06 ± 57.28^b	288.66 ± 43.10^ab	270.17 ± 45.34^ab	0.21	0.65	8.21	< 0.01
Threonine	41.69 ± 8.24^a	52.61 ± 9.06^ab	60.30 ± 12.22^b	56.40 ± 10.53^b	51.20 ± 4.70^ab	52.12 ± 10.69^ab	1.82	0.19	8.09	< 0.01
Methionine	1.13 ± 0.44^a	1.78 ± 0.66^abc	2.38 ± 1.38^bc	2.88 ± 0.57^c	2.21 ± 0.82^abc	1.68 ± 1.23^ab	2.08	0.16	10.32	< 0.01
Valine	5.49 ± 1.54^ab	5.59 ± 1.53^ab	5.31 ± 0.71^a	7.31 ± 1.22^bc	8.61 ± 1.13^c	8.58 ± 2.42^c	26.06	< 0.01	1.00	0.32
Isoleucine	2.64 ± 0.45^a	2.78 ± 0.75^a	2.77 ± 0.39^a	3.05 ± 0.65^a	4.99 ± 1.24^b	4.82 ± 1.41^b	33.60	< 0.01	3.42	0.07
Phenylalanine	3.19 ± 0.79^b	2.58 ± 0.71^ab	2.32 ± 0.71^a	4.28 ± 0.86^b	4.41 ± 0.86^b	4.96 ± 0.77^b	36.88	< 0.01	6.27	< 0.05
Leucine	15.24 ± 2.48^ab	13.24 ± 2.95^a	12.96 ± 4.10^a	16.74 ± 2.73^ab	19.67 ± 3.91^b	19.58 ± 4.51^b	13.80	< 0.01	2.88	0.10
Tyrosine	4.48 ± 0.71^c	2.11 ± 0.61^a	2.21 ± 0.51^a	2.70 ± 0.68^ab	3.20 ± 1.04^b	3.39 ± 0.98^b	0.40	0.53	24.44	< 0.01
Arginine	57.46 ± 13.40^ab	93.46 ± 33.77^b	95.85 ± 56.11^b	76.72 ± 32.40^ab	57.47 ± 8.92^ab	43.43 ± 13.02^a	3.47	0.07	8.43	< 0.01
Serine	16.56 ± 3.54	14.19 ± 2.91	14.86 ± 5.71	13.32 ± 3.42	13.19 ± 4.42	12.53 ± 3.03	3.33	0.08	0.13	0.72
Lysine	50.46 ± 8.98^a	55.37 ± 15.72^a	69.94 ± 17.71^a	104.92 ± 26.61^b	116.01 ± 27.05^b	154.55 ± 24.59^c	104.12	< 0.01	3.80	0.06
Histidine	446.84 ± 67.81^a	547.10 ± 73.00^b	557.44 ± 49.11^b	621.42 ± 82.94^bc	661.77 ± 51.30^c	566.86 ± 80.47^b	18.45	< 0.01	11.08	< 0.01
TAA	1225.27 ± 75.89^a	1414.85 ± 149.59^b	1393.32 ± 146.26^b	1466.31 ± 107.43^b	1443.14 ± 55.46^b	1344.20 ± 107.23^ab	3.85	0.06	11.90	< 0.01
IMP	48.03 ± 4.82^a	56.24 ± 3.74^bc	57.67 ± 5.05^bc	61.01 ± 5.48^c	51.88 ± 5.94^ab	51.22 ± 6.05^ab	0.12	0.73	19.53	< 0.01

Values are means ± SD and n = 6 for each group. ^a-c Different superscripts in the same row indicate significantly difference (P < 0.05), Phenylalanine (P < 0.08)