. 2022 Aug 25;13:109. doi: 10.1186/s40104-022-00747-7

Table 6.

The shear force, Hydroxyproline, collagen and IGF-1 contents, and collagen metabolism-related enzyme activities in muscle

Items	Dietary protein levels						Linear		Quadratic
Items	15.91%	19.39%	22.10%	25.59%	28.53%	31.42%	F	P	F	P
Shear force, N	2.13 ± 1.00^ab	3.61 ± 0.66^c	3.30 ± 0.45^c	2.81 ± 0.54^bc	1.86 ± 0.34^a	2.12 ± 0.73^ab	6.68	< 0.05	12.55	< 0.01
Hyp, μg/mg	0.31 ± 0.02^a	0.34 ± 0.04^b	0.60 ± 0.02^e	0.45 ± 0.01^d	0.46 ± 0.02^d	0.39 ± 0.02^c	64.14	< 0.01	288.66	< 0.01
Collagen, μg/mg	2.44 ± 0.12^a	2.69 ± 0.31^b	4.80 ± 0.20^e	3.64 ± 0.11^d	3.68 ± 0.16^d	3.14 ± 0.19^c	64.13	< 0.01	288.56	< 0.01
IGF-1, ng/mL	29.73 ± 2.18^a	30.31 ± 1.74^a	32.95 ± 1.51^ab	33.90 ± 2.76^b	33.57 ± 2.73^b	30.04 ± 4.05^a	1.87	0.18	10.87	< 0.01
LOX, ng/mL	12.76 ± 0.81^a	13.71 ± 0.84^ab	15.23 ± 1.43^b	14.72 ± 1.43^b	14.65 ± 1.62^b	14.06 ± 1.28^ab	4.11	0.05	8.76	< 0.01
P4H, μg/L	78.57 ± 6.39^a	83.05 ± 6.56^ab	88.46 ± 5.55^b	84.95 ± 5.31^ab	83.40 ± 7.32^ab	83.28 ± 1.75^ab	1.15	0.29	5.55	< 0.05
MMP2, ng/mL	88.70 ± 1.81^ab	85.50 ± 3.18^ab	84.22 ± 6.01^a	88.12 ± 2.88^ab	89.25 ± 10.21^ab	92.37 ± 5.97^b	2.92	0.10	3.69	0.46

Values are means ± SD and n = 6 for each group. ^a-e Different superscripts in the same row indicate significantly difference (P < 0.05)