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. 2022 Aug 24;22:502. doi: 10.1186/s12887-022-03570-1
Region Primers
V3-V4 [17]

341F (5'-CCTACGGGNGGCWGCAG-3')

805R(5'-GACTACHVGGGTATCTAATCC-3')

Archae [18]

F(5'-GYGCASCAGKCGMGAAW-3')

R(5'-GGACTACHVGGGTWTCTAAT-3')

V4 [19]

515F(5'-GTGYCAGCMGCCGCGGTAA-3')

806R (5'- GGACTACHVGGGTWTCTAAT-3')

V4-V5

F(5'-GTGCCAGCMGCCGCGG-3')

R(5'-CCGTCAATTCMTTTRAGTTT-3')