Table 2.
Primers used in this study.
| Primer | DNA sequence (5′–3′)a | Purpose |
|---|---|---|
| 27F | 5´-AGAGTTTGATCCTGGCTCAG-3´ | Amplification of the 16S rRNA gene of the dicamba degrader |
| 1492R | 5´-GGTTACCTTGTTACGACTT-3´ | |
| pET-dmt06-F | TAAGAAGGAGATATACATATGGGAGAAGGACGGTCCCTTCA | Amplification of dmt06 gene for expression in E. coli BL21 |
| pET-dmt06-R | AGTGCGGCCGCAAGCTTCGGCGACCCGGCGGCCGTCGCC | |
| dF1 | CTC(G)TTCG(A)ACCAGT(A)CC(G)CACCACATG | Amplification of the conserved regions of dmt06 gene |
| dF2 | TCGGCGACT(G)GC(G)ATCCTG(T)TAC(T)TA(G) | |
| dR1 | GGC(A)TGGATC(G)C(G)CC(G)TACCCG(C)CTG(C)GCCG | |
| dR2 | TC(G)AAGTTC(T)GAC(T)CAC(T)GACTTCATCGG | |
| uSP1 | AGTACACGATGCAGTCGCCGACCACGT | Amplification of the upstream sequence of dmt06 |
| uSP2 uSP3 |
AACCCGGCGAAGGTGTTGATGCCGA CTTTCAGGAACAGCTNNNNNNNNNGGTGGG |
|
| dSP1 | AGTGGAGCTGTCCGGCCCGTAC | Amplification of the downstream sequence of dmt06 |
| dSP2 | GACACCGTACGGTCGGCCATTCTC | |
| dSP3 | AGAAGTACGGAATCGNNNNNNNNNGCACCC |
a
The underlined bases indicate that they were overlapped and were used to construct plasmids by the In-Fusion.