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. 2022 Aug 11;13:958739. doi: 10.3389/fmicb.2022.958739

FIGURE 2.

FIGURE 2

Adenoids and tonsils have similar frequency of adaptive and innate immune cells. Adenoids and tonsils were obtained from PLWH and SN. The frequency (%) of adaptive and innate immunity cells were assessed by flow cytometry. (A) The frequencies (%) of T cells (CD3+), B cells (CD19+), and CD4+ T cells subpopulations: Tfhs (CD3+CD4+CD45RO+CXCR5+), Tregs (CD3+CD4+CD45RO+CD25+FOXP3+), Th1 (CD3+CD4+CD45RO+T-bet+), Th2 (CD3+CD4+CD45RO+CRTH2+), and Th17 (CD3+CD4+CD45RO+RORgt+) are shown. (B) The frequencies (%) of NK cells (CD56+), macrophages (classical CD14- CD16+, non-classical CD14+ CD16- and inflammatory CD14+ CD16+ HLADR-) and dendritic cells (myeloid dendritic cells CD123- CD11c+ and plasmacytoid dendritic cells CD123+ CD11c-) are shown. Scatter plots were used to present the data. The median is shown by a horizontal line. Each symbol represents one individual. Red triangle: PLWH tonsils, red circle: PLWH adenoids, blue triangle: SN tonsils, and blue circle: SN adenoids. Data was compared using the Wilcoxon Rank Sum test. Only p values < 0.05 are shown in the graphs. *p < 0.01; **p < 0.001. %, frequency; CM, classical macrophages; CRTH2, chemoattractant receptor-homologous molecule expressed on TH2 cells; FOXP3, forkhead box P3 protein; HIV, human immunodeficiency virus; IM, inflammatory macrophages; mDC, myeloid dendritic cells; pDC, plasmacytoid dendritic cells; PLWH, people living with HIV; NCM, non-classical macrophages; NK, natural killer; RORgt, retinoic acid-related orphan receptor-gammat; SN, seronegative; Tfhs, T follicular helper cells; Th, T helper; and Tregs, T regulatory.