Figure 5.

(A) Synthesis of a combinatorial polymer–TLR agonist library comprised by postpolymerization modification of a thiazolidine-containing, water-soluble polymeric scaffold. (B) Polymers with varied charge, TLR agonist density, and linker length were prepared. (C) IL-12p40 secreted by lymph node-derived cells 24 h after injection was then used to screen immunostimulatory activity of the polymers. Polymers that self-assemble into nanoparticles were found to maximize IL-12p40 secretion. Reproduced with permission from ref (7). Copyright 2015 Springer Nature.