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. 2022 Aug 11;13:927763. doi: 10.3389/fendo.2022.927763

Figure 3.

Figure 3

ANGPTL8 deletion suppresses diet-induced ectopic lipid deposition in the liver, kidney, heart, and WAT of male mice. (A) Micrographs showing Oil Red O staining of the frozen liver, kidney, and heart sections from male WT and ANGPTL8 KO mice after NCD feeding for 20 months (red = Oil Red O, scale bar = 50 μm, n = 5). (B) Micrographs showing Oil Red O staining of frozen liver, kidney, and heart sections from male WT and ANGPTL8 KO mice after HFD consumption for 20 months (red = Oil Red O, scale bar = 50 μm, n = 5). (C) Micrographs showing Sudan Black B staining of frozen liver, kidney, and heart sections from male WT and ANGPTL8 KO mice after NCD feeding for 20 months (scale bar = 50 μm, n = 5). (D) Micrographs showing Sudan Black B staining on frozen liver, kidney, and heart sections in male WT and ANGPTL8 KO mice after HFD consumption for 20 months (scale bar = 50 μm, n = 5). (E) Quantitative analysis of TG in the liver, kidney, and heart of ANGPTL8-KO and WT male mice treated with NCD for 20 months. (F) Quantitative analysis of TG in the liver, kidney, and heart of ANGPTL8-KO and WT male mice fed an HFD for 20 months. (G) Representative HE staining of white adipose tissue of male WT and ANGPTL8 KO mice after NCD for 20 months (scale bar = 50 μm, n = 5). (H) Representative HE staining of white adipose tissue of male WT and ANGPTL8 KO mice after HFD consumption for 20 months (scale bar 50 = μm, n = 5).