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. 2022 Aug 19;11(8):1611. doi: 10.3390/antiox11081611

Figure 2.

Figure 2

SIRT3+ reduced the PA-related cytotoxicity. (A) Immunoblotting analysis of SIRT3 protein levels, (B) LDH assay cytotoxicity, (C) MDA, and (D) extracellular ROS content in EC treated with the empty transfection reagent (Lullaby) or transfected with mimic Negative Control (mimic NC), SIRT3 mimic (SIRT3+), mimic Negative Control and then exposed to 0.5 mM PA for 48 h (mimic NC+PA) or SIRT3 mimic before 48 h treatment with PA (SIRT3++PA). Control cells (Ctr) were treated with the corresponding volume of HBSS-10 mM Hepes. Western blotting results (n = 5) are expressed as arbitrary units (AU) and represented as boxplots. ‡ p < 0.001 vs. mimic NC; § p < 0.05 vs. mimic NC+PA; # p < 0.01 vs. mimic NC+PA.