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. 2022 Jul 29;11(8):1487. doi: 10.3390/antiox11081487

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Western blot analysis and relative density changes for the KGDHc subunits, normalized for superoxide dismutase 2 (SOD2) protein expression, in brain mitochondria isolated from the wild-type and KGDHc-subunit-KO mice. (A) alpha-ketoglutarate/2-oxoglutarate (KGDH/OGDH) subunit; (B) dihydrolipoyl succinyltransferase (DLST) subunit; (C) dihydrolipoyl dehydrogenase (DLD) subunit. White bars: wild-type (WT); bars with left diagonal stripes: dihydrolipoyl succinyltransferase mutation (DLST^+/−); bars with right diagonal stripes: dihydrolipoyl dehydrogenase mutation (DLD^+/−). Results are expressed as means of the relative densities ± S.E.M. (N = 3–4). Statistically significant differences are indicated by asterisks; * p < 0.05.