Table 1.
Primers used for quantitative polymerase chain reaction (qPCR) analyses.
| Target Genes (Accession n.) |
Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| PPIA (NM_021130.5) |
CTGCACTGCCAAGACTGA | GCCATTCCTGGACCCAAA |
| GSS (NM_000178.4) |
GCGGAGGAAAGGCGAACTA | AGAGCGTGAATGGGGCATAG |
| GCLC (NM_001498.4) |
ACGGAGGAACAATGTCCGAG | TCCACTGGGTTGGGTTTGAC |
| GSR (NM_000637.5) |
GTGGAGGTGCTGAAGTTCTCC | AACCATGCTGACTTCCAAGC |
| GPX4 (NM_002085.5) |
CCTGGACAAGTACCGGGGC | CTTCGTTACTCCCTGGCTCCTG |
| xCT (NM_014331.4) |
TGAAATCCCTGAACTTGCGAT | TCTGGATCCGGGCGCT |
| TXNRD1 (NM_182729.3) |
CGATCTGCCCGTTGTGTTTG | TATTGGGCTGCCTCCTTAGC |
| CHAC1 (NM_024111.6) |
TTCTGGCAGGGAGACACCTT | GCCTCTCGCACATTCAGGTA |
| SLC25A11 (NM_003562.5) |
CGTCAAGTTCCTGTTTGGGG | AGTGTAAATGCCCCTCAGGC |
PPIA: cyclophilin A; GSS: glutathione synthetase; GCLC: glutamate-cysteine ligase catalytic subunit; GSR: glutathione-disulfide reductase; GPX4: glutathione peroxidase 4; xCT: solute carrier family 7 member 11 (SLC7A11); TXNRD1: thioredoxin reductase 1; CHAC1: ChaC glutathione specific gamma-glutamylcyclotransferase 1; SLC25A11: solute carrier family 25 member 11.