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. 2022 Aug 13;11(8):1567. doi: 10.3390/antiox11081567

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of ZnO-NPs on necroptosis via mutated tP53 formation: (A) Western blot analysis of the tP53 protein, Vasa protein, and Pgr protein normalized to β-actin in control, T1, and T2 groups. (B) Western blot analysis of procaspase-3 and cleaved caspase-3 in the control, T1, and T2 groups revealed the overexpression of cleaved caspase-3 in T2 compared with the control and T1 groups. (C–F) Quantified protein expression revealed the ratio analysis of tP53/β-actin, Vasa/β-actin, Pgr/β-actin, and cleaved -cas3/pro-cas3, respectively quantified by the Image J software. Data expressed as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. T1 = group was exposed to 1/5th of the estimated LC₅₀ of ZnO-NPs in water daily for two weeks (15 days). T2 = group was exposed to 1/5th of the estimated LC₅₀ of ZnO-NPs in water daily for one month (30 days).