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CuSO4 does not alter expression from the PY promoter. A PY-lacZ strain (PAS3) was grown to log phase, after which the culture was split and 3 mM CuSO4 added (open symbols) or not added (closed symbols). OD600 is shown as gray squares and β-galactosidase activity as black circles. Symbols represent the mean, and the error bars are the standard error of the mean (SEM) of 2 biological replicates.
