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. 2022 Aug 10;11(8):1085. doi: 10.3390/antibiotics11081085

Figure 1.

Figure 1

Antibiofilm and antipersister activity of designed LfcinB6 peptides against E faecium strain C68. (A) Inhibition of biofilm formation by peptides 5L–8L, live-cell reductions by XTT and (B) biomass by CV. (C) Disruption of 24 h established biofilms, live-cell reductions by XTT, and (D) biomass by CV. (E) Confocal microscopy of control E. faecium biofilm and (F) 5L- and (G) 6L-treated biofilms at 32 µg/mL. (H) Key biofilm genes regulation by peptides 5L and 6L via real-time PCR (significant, * p < 0.05, determined by Student’s t-test) (I) The kinetic killing of E. faecium strain C68 persister cells by 5L and 6L at 10× MIC. (J) SYTOX-based membrane permeabilization of persister cells by peptides 5L and 6L at 16 µg/mL. (K) The kinetic killing of E. faecium strain C68 exponential cells by 5L and 6L at 10× MIC. (L) SYTOX-based membrane permeabilization of exponential cells by peptides 5L and 6L at 16 µg/mL.