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Fixative solution
| Reagent | Final concentration | Amount |
|---|---|---|
| PFA 16% | 4% (w/v) | 10 mL |
| GA 25% | 0.2% (v/v) | 320 μL |
| PB (pH 7.4) 0.2 M | 0.1 M | 20 mL |
| ddH2O | n/a | up to 40 mL |
| Total | n/a | 40 mL |
Filter the solution through filter paper.
Use the fixative solution within the day.
The final concentration of GA can be changed from 0.02% to 2% depending on experiments.
