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Expression of genes and proteins related to OPN5 and TSH-DIO2/DIO3 pathway. (A)The relative expression of hypothalamic OPN5 mRNA. (B–E) The relative expression of hypothalamic THRβ, DIO2, DIO3, TRH mRNA, respectively. (F, G) The relative expression of TSHβ and TSHR mRNA of pituitary. (H, I) Western blot analysis of OPN5, THR, DIO2 and DIO3 protein expression of hypothalamus.
