Table 1.
Pimers used in the real-time quantitative PCR assay of the genes.
| Gene name | Primer sequence (5´-3´) | Annealing temperature (°C) | PCR products (bp) | Accession number |
|---|---|---|---|---|
| OPN5 | F: ACCAGGATCCAGAACAGCCA R: GCAATGAGGAATCCGGCACA |
60 | 80 | KM922545.1 |
| TRH | F:TGGTGAAGTAAATTGCCAGAACAC R: CCTAAATGGGGACACTCACTCAC |
60 | 106 | XM_013182272.1 |
| THRβ | F: GCTTATCTCTGGGCAATGTGAC R: TTGAAGCGACATTCCTGGCA |
60 | 299 | XM_038174583.1 |
| TSHβ | F:CGTGTGCACATACAAAGAGAT R: GCAATAGTTTGGCCTAACCTT |
60 | 162 | NM_001310425.1 |
| TSHR | F: TGTTAACTGCCTATGCGCCA R: CCGGTCAGGATGGTAGAAGC |
60 | 200 | XM_009646634.2 |
| DIO2 | F:GACGCCTACAAGCAGGTCAA R: GTTCCACACTTGCCACCAAC |
57 | 119 | XM_013194618.1 |
| DIO3 | F:TGGGCTTAAAGAGAAGCGGG R: CTAGGAGCCAGAGCATCACG |
57 | 214 | XM_013199473.1 |
| GnRH | F: CTGGGACCCTTGCTGTTTTG R: AGGGGACTTCCAACCATCAC |
59 | 209 | NM_001080877.1 |
| GnIH | F: AAAGTGCCAAATTCAGTTGCT R: GCTCTCTCCAAAAGCTCTTCC |
58 | 128 | XM_015853673.2 |
| VIP | F: CTGGGAAACAGACTGCCCTT R: GACTGTCCTGGGAGCTATCAAT |
56 | 132 | XM_013178195.1 |
| FSHβ | F: GTGGTGCTCAGGATACTGCTTCA R: GTGCAGTTCAGTGCTATCAGTGTCA |
60 | 209 | XM_031607398.1 |
| LHβ | F: CCAGGCCTCCTGCACCTAC R: GGCGCAGCGGCAGCTCAG |
60 | 115 | MK820637.1 |
| PRL | F: ACCTCCTTGCCTATCTGCCC R: TTGTAATGAAACCCCGACCC |
60 | 136 | XM_013184821.1 |
| β-actin | F: CCTCTTCCAGCCATCTTTCTT R: TGTTGGCATACAGGTCCTTAC |
56 | 110 | XM_035563367.1 |