Table 2.

Protocols to generate subtype-defined hPSC-CMs

Pink cells indicate positive inducers (growth factors, sera, small molecules); yellow cells indicate inhibitors; green cells indicate media and additional supplements used. Acronyms: 1TG monothioglycerol; BMP4, bone morphogenetic protein 4; BPEL, Bovine Serum Albumin (BSA) Polyvinylalchohol Essential Lipids medium containing IMDM/F12, lipids, Protein-Free Hybridoma mixture II, insulin, transferrin, sodium selenite, ascorbic acid, L-alanyl-l-glutamine, penicillin. streptomycin; DKK1, Dickkopf-related protein-1; EB, embryoid body; FGF2, basic fibroblast growth factor; HSA, human serum albumin; IWP-2, inhibitor of Wnt production 2; MEF-CM, MEF conditioned medium; VEGFA, vascular endothelial growth factor.