Table 5.
Viral packaging for direct reprogramming
| Cells | Overexpressed genes | Virus | Backbone | Transfection | Polybrene | Positive selection | Induction system | Reference |
|---|---|---|---|---|---|---|---|---|
| Platinum E cells | GMT | Retrovirus | pMXs | FuGene6 (Roche) | leda et al., 2010 | |||
| Platinum E cells | Myc-tagged GHMT | Retrovirus | pBabeX | FuGene6 (Roche) | 6 μg/mL | Song et al. (2012) | ||
| HEK293 FT | GMT, ESRRG, MESP1, MYOCD, ZFPM2 (7F) | Lentivirus | pLVX-tetO-cDNA | Lipofectamine 2000 | 4 μg/mL | 1 μg/mL Dox | Fu et al. (2013) | |
| pLVX-Tight-Puro | ||||||||
| Retrovirus | pMXs | FuGene6 (Promega) | ||||||
| Retro-X-Tet-ON | 5 μg/mL | puromycin & neomycin | ||||||
| pRetro-X-Tight-cDNA | 100 ng/mL Dox | |||||||
| Platinum A cells | GMT, Mesp1, Myocd | Retrovirus | pBabeX | FuGene6 (Promega) | 6 μg/mL | Nam et al. (2013) | ||
| PLAT-GP cells | GMT, MESP1, MYOCD (GMTMM) | Retrovirus | pMXs | TremeGene 9 (Roche) | 4 μg/mL | Wada et al. (2013) | ||
| 293FT cells | Lentivirus | pLVX-Tight-Puro | Lipofectamine 2000 | 4 μg/mL | 1 μg/mL Dox | |||
| pLVX-rtTA | ||||||||
| pLVX-tetO-cDNA | ||||||||
| Platinum A cells | Myc-tagged GHMT | Retrovirus | pBabeX | FuGene6 (Promega) | 6 μg/mL | Nam et al. (2014) | ||
| Platinum E cells | GMT | Retrovirus | pMXs | FuGene6 (Roche) | Muraoka et al. (2014) | |||
| HEK293 | human GMT, MESP1, MYOCD | Lentivirus | CSII-CMV-RfA | Lipofectamine 2000 | ||||
| Platinum E cells | Polycistronic M-G-T | Retrovirus | pMXs | Lipofectamine 2000 | 4 μg/mL | puromycin 2 μg/mL | Wang et al. (2015a) | |
| Platinum A cells | GHMT | Retrovirus | pBabeX | FuGene6 (Promega) | 6 μg/μL | Zhou et al. (2015) | ||
| Akt1 | pMxs-MyrAkt1 | |||||||
| Platinum E cells | GHMT, MESP1, MYOCD | Retrovirus | pMXs | FuGene6 (Promega) | Yamakawa et al. (2015) | |||
| Platinum E cells | GMT | Retrovirus | pMXs | FuGene HD (Roche) | 0.6 μg/mL | Mohamed et al. (2017) | ||
| Platinum E cells | GMT or GHMT | Retrovirus | pMXs | FuGene6 | 10 μM diclofenac | Muraoka et al. (2019) | ||
| Platinum E cells | polycistronic M-G-T-H | Retrovirus | pBabeX | FuGene6 (Promega) | 6 μg/mL | Zhang et al. (2019a)(2021c) | ||
| 293T cells | Polycistronic human M-G-T, miRNA-133 | Retrovirus | pMXs | Nanofect | 8 μg/mL | puromycin 1 μg/mL | Garbutt et al. (2020) | |
| pBabe | ||||||||
| Platinum E cells | GHMT, Myocd, PHF7 | Retrovirus | pMXs | FuGene6 (Promega) | 8 μg/mL | Garry et al. (2021) |
Acronyms: Dox, doxycycline.