FIG. 1.

Effects of different carbon sources on expression of OprD. (A) SDS-PAGE analysis of outer membrane proteins from P. aeruginosa PAO1; (B) Western immunoblot of outer membrane proteins probed with an OprD-specific monoclonal antibody. Molecular weight standards: (A) 94,000, 67,000, and 43,000; (B) 81,000, 47,700, and 34,600. The positions of OprD, OprB, and OprF are indicated by arrows on the left. Lane 1, 0.4% glucose; lane 2, 20 mM gluconate; lane 3, 1% glycerol; lane 4, 20 mM succinate; lane 5, 20 mM arginine; lane 6, 20 mM glutamate; lane 7, 20 mM histidine; lane 8, 20 mM alanine; lane 9, 4 mM gluconate; lane 10, 4 mM succinate.