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. 1999 Sep;181(17):5426–5432. doi: 10.1128/jb.181.17.5426-5432.1999

FIG. 4.

FIG. 4

ArgR-dependent induction of OprD, determined by SDS-PAGE analysis of outer membrane proteins isolated from strains PAO1 and PAO501 (argR deficient). All cultures contained ammonium sulfate as the nitrogen source and carbon sources as indicated. Lane 2, PAO1 with succinate (20 mM); lane 3, PAO501 with succinate (20 mM); lane 4, PAO1 with glutamate (20 mM); lane 5, PAO501 with glutamate (20 mM); lane 6, PAO501 with 1% glycerol and arginine (20 mM); lane 7, PAO501 with 1% glycerol; lane 8, PAO1 with 1% glycerol and arginine (20 mM); lane 9, PAO1 with 1% glycerol. Molecular standards are, from top to bottom, 104,000, 81,000, 47,700, and 34,600 in lane 1 and 94,000, 67,000, 43,000, and 30,000 in lane 10. The positions of OprD and OprF are indicated by arrows.