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. 2022 Aug 8;11(8):1539. doi: 10.3390/antiox11081539

Figure 6.

Figure 6

Knockdown of NOX1 or NOX4 in mice attenuated ACS induced upregulation of pro-inflammatory mediators in the mouse lung. Mice were exposed to CS or filtered air for 1 h and harvested at 6 h. The mRNA expression levels of (A,B) TNF-α and (C,D) KC in the lungs of ACS exposed mice silenced or knocked down of NOX1, NOX2, or NOX4 were determined by RT-qPCR. The data indicate that ACS induced upregulation of TNF-α/KC in mouse lungs was significantly attenuated in NOX1- or NOX4-silenced mice, while it was increased in NOX2−/y mice. All data are presented as Mean ± SEM, n = 4–5. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. air exposed control group. # p < 0.05, ### p < 0.001 vs. ACS exposed group.