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. 2022 Aug 8;11(8):1539. doi: 10.3390/antiox11081539

Figure 7.

Figure 7

Knockdown of NOX1 or NOX4 attenuated ACS induced NF-κB activation in the mouse lung. Mice were exposed to CS or filtered air for 1 h and harvested at 6 h. Shown are representative immunohistochemical images of p65 (A,B) and p-p65 (C,D) expression in ACS exposed mice silenced or knocked out of NOX1, NOX2, or NOX4. Quantitative data of p65 and p-p65 expression in ACS exposed mice silenced or knocked out of NOX1, NOX2, or NOX4 are shown in panels (E,F), respectively. The data indicate that ACS induced activation of NF-κB was significantly attenuated in NOX1- or NOX4-silenced mice, while it was increased in NOX2−/y mice. All data are presented as Mean ± SEM, n = 6. Scale bar, 50 µm. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. air exposed control group. # p < 0.05, ## p < 0.01 vs. ACS exposed group.