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. 2022 Aug 8;11(8):1539. doi: 10.3390/antiox11081539

Figure 8.

Figure 8

Knockdown of NOX1 or NOX4 attenuated ACS induced activation of the NF-κB-COX-2 pathway in the mouse lung. Mice were exposed to CS or filtered air for 1 h and harvested at 6 h. Western blotting was used to examine COX-2, p-IKKα/β, p-p65, p65, and IκBα protein expression in mouse lungs. (A,B) Representative Western blots of COX-2, p-IKKα/β, p-p65, p65, and IκBα protein expression in ACS exposed mice silenced or knocked out of NOX1, NOX2, or NOX4. (C) Grouped data of COX-2, p-IKKα/β, p-p65, p65, and IκBα protein expression in ACS exposed mice silenced or knocked out of NOX1, NOX2, or NOX4. The data indicate that ACS induced activation of the NF-κB-COX-2 pathway was significantly attenuated in NOX1- or NOX4-silenced mice, while it was increased in NOX2−/y mice. All data are presented as Mean ± SEM, n = 3–5. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. air exposed control group. # p < 0.05, ## p < 0.01 vs. ACS exposed group.