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. 2022 Jul 29;10(8):1835. doi: 10.3390/biomedicines10081835

Figure 4.

Figure 4

pFAKY397 and FAK expression in cancer cell lines and normal fibroblasts cultured in RAD16-I hydrogels. (a) Western blot bands of pFAKY397 and total FAK in PANC-1, MiaPaCa-2 and hNDF cells cultured in 2D and 0.15% (soft) and 0.5% (stiff) RAD16-I gels; (b) densitometry of bands shown in (a); (c) Western blot bands of pFAKY397 and total FAK in PANC-1 cells cultured in 3D RAD16-I hydrogels of increased stiffness (from 0.15% to 0.8% RAD16-I, representing a stiffness range of 100–8500 Pa); (d) densitometry of bands shown in (c). GAPDH was used as loading control. Statistical differences are indicated as * for p value < 0.05, ** for p value < 0.01, *** for p value < 0.001 and **** for p value < 0.0001.