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. 2022 Jul 29;10(8):1835. doi: 10.3390/biomedicines10081835

Figure 5.

Figure 5

Cell culture in RAD16-I matrix functionalized with collagen type I. (a) SDS-PAGE of RAD or RAD/COL cell-free gels; (b) phase-contrast microscopy images of fibroblast 24 h after cell embedding within 0.15% RAD or RAD/COL gels. Scale bars represent 50 µm; (c) Western blot bands of FAK and Erk1/2 and its phosphorylated forms in PANC-1 cells cultured in 0.15% (soft) and 0.5% (stiff) RAD16-I gels mixed with increasing amounts of collagen I; (d) densitometry of pFAKY397/FAK shown in (c). GAPDH was used as loading control. Statistical differences are indicated as ** for p value < 0.01, *** for p value < 0.001 and **** for p value < 0.0001.