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Cell culture in 3D collagen type I gels. (a) Macroscopic view of collagen 3D constructs containing PANC-1 cells; (b) Western blot bands of pFAKY397 and total FAK in PANC-1, MiaPaCa-2 and MCF-7 cells cultured in 0.15% (soft) and 0.5% (stiff) collagen gels; (c) densitometry of bands shown in (b) represented as pFAKY397/FAK; (d) Western blot bands of pFAKY397 and total FAK in PANC-1 cells cultured in 0.15% (soft) and 0.5% (stiff) collagen gels in the presence of GM6001.
