. 2022 Aug 17;12(16):2100. doi: 10.3390/ani12162100

Table 3.

Primers used in the qPCR assay.

Target	Primer	Sequence (5′→3′)	Annealing Temperature (°C)	Reference
Blautia spp.	Blautia_F	TCTGATGTGAAAGGCTGGGGCTTA	62.0	[44]
Blautia spp.	Blautia_R	GGCTTAGCCACCCGACACCTA	62.0	[44]
Turicibacter spp.	Turicibacter_F	CAGACGGGGACAACGATTGGA	59.3	[44]
Turicibacter spp.	Turicibacter_R	TACGCATCGTCGCCTTGGTA	59.3	[44]
Ruminococcaceae	Ruminococcaceae_F	ACTGAGAGGTTGAACGGCCA	64.2	[45]
Ruminococcaceae	Ruminococcaceae_R	CCTTTACACCCAGTAAWTCCGGA	64.2	[45]
Bifidobacterium spp.	Bif_F	TCGCGTCYGGTGTGAAAG	62.0	[46]
Bifidobacterium spp.	Bif_R	CCACATCCAGCRTCCAC	62.0	[46]
Lactobacillus spp.	Lac_F	AGCAGTAGGGAATCTTCCA	64.2	[47]
Lactobacillus spp.	Lac_R	CACCGCTACACATGGAG	64.2	[47]
Clostridium leptum	sg-Clept-F	GCACAAGCAGTGGAGT	59.3	[48]
Clostridium leptum	sg-Clept-R	CTTCCTCCGTTTTGTCAA	59.3	[48]
Clostridium coccoides	g-Ccoc-F	AAATGACGGTACCTGACTAA	64.2	[49]
Clostridium coccoides	g-Ccoc-R	CTTTGAGTTTCATTCTTGCGAA	64.2	[49]
Clostridium hiranonis	C.hiranonis_F	AGTAAGCTCCTGATACTGTCT	65.4	[50]
Clostridium hiranonis	C.hiranonis_R	AGGGAAAGAGGAGATTAGTCC	65.4	[50]
Escherichia coli	Coli_F	GTTAATACCTTTGCTCATTGA	62.0	[51]
Escherichia coli	Coli_R	ACCAGGGTATCTAATCCTGTT	62.0	[51]
Enterococcus spp.	Ent_F	CCCTTATTGTTAGTTGCCATCATT	59.3	[46]
Enterococcus spp.	Ent_R	ACTCGTTGTACTTCCCATTGT	59.3	[46]
Clostridium cluster XIV	CloXIV-F	GAWGAAGTATYTCGGTATGT	57.2	[52]
Clostridium cluster XIV	CloXIV-R	CTACGCWCCCTTTACAC	57.2	[52]