Table 4.

pH values, ammonia and short-chain fatty acids concentrations after 6 h of an in vitro incubation of canine fecal inoculum supplemented with microalgae ¹.

Item	CTRL	AP	HP	PT	CV	Pooled SEM	Anova p-Value
pH	6.71	6.63	6.58 *	6.63 *	6.56 *	0.03	0.005
Ammonia, mmol/L	30.2	32.2	31.4	29.6	31.9	1.62	0.586
Straight-chain SCFA, mmol/L
Acetate	8.62	8.66	8.97	8.85	8.57	0.42	0.954
Propionate	4.54	4.92	5.13	6.19 *	5.14	0.23	0.001
Butyrate	2.55	2.58	2.62	3.16 *	2.69	0.12	0.013
Total SCFA	15.7	16.2	16.7	18.2	16.4	0.78	0.232
BCFA, mmol/L
Isobutyrate	0.27	0.15	0.15	0.13 *	0.13	0.03	0.022
Isovalerate	0.46	0.26 *	0.30	0.26 *	0.26 *	0.03	0.009
Total BCFA	0.73	0.41	0.45	0.39 *	0.39 *	0.08	0.006
Individual SCFA proportions, %
Acetate	51.5	52.2	52.1	47.6 *	51.0	0.44	<0.001
Propionate	27.1	29.7 *	29.9 *	33.3 *	30.6 *	0.23	<0.001
Butyrate	15.2	15.5	15.2	17.0 *	16.0 *	0.15	<0.001
Isobutyrate	1.54	0.88	0.90	0.71 *	0.80 *	0.13	0.001
Isovalerate	2.70	1.59	1.74	1.42 *	1.56 *	0.13	<0.001

¹ Values are the means of five bottles per treatment. * Significantly different from CTRL, p < 0.05. CTRL, control; AP, Arthrospira platensis; HP, Haematococcus pluvialis; PT, Phaeodactylum tricornutum; CV, Chlorella vulgaris; SCFA, short-chain fatty acid; BCFA, branched-chain fatty acid.