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. 2022 Aug 17;12(16):2100. doi: 10.3390/ani12162100

Table 5.

pH values, ammonia and short-chain fatty acids concentrations after 24 h of an in vitro incubation of canine fecal inoculum supplemented with microalgae 1.

Item CTRL AP HP PT CV Pooled SEM Anova
p-Value
pH 5.84 5.84 5.81 5.95 5.81 0.01 0.004
Ammonia, mmol/L 39.6 39.9 36.0 38.0 35.7 1.29 0.065
Straight-chain SCFA, mmol/L
Acetate 16.7 16.9 16.6 16.5 16.5 0.48 0.960
Propionate 9.68 10.5 10.3 11.7 * 10.7 0.28 0.001
Butyrate 5.43 5.73 5.31 5.65 5.61 0.14 0.271
Total SCFA 31.8 33.1 32.2 33.8 32.8 0.89 0.536
BCFA, mmol/L
Isobutyrate 0.60 0.64 0.60 0.64 0.62 0.02 0.289
Isovalerate 0.92 0.95 0.90 1.01 * 0.94 0.02 0.041
Total BCFA 1.52 1.59 1.50 1.65 1.56 0.04 0.086
Individual SCFA proportions, %
Acetate 48.7 47.3 * 47.9 * 45.2 * 46.7 * 0.20 <0.001
Propionate 28.3 29.4 * 29.7 * 32.2 * 30.2 * 0.09 <0.001
Butyrate 15.9 16.0 15.4 * 15.6 15.9 0.10 0.001
Isobutyrate 1.76 1.80 1.74 1.76 1.75 0.01 0.041
Isovalerate 2.68 2.66 2.59 2.78 2.65 0.05 0.254

1 Values are the means of five bottles per treatment. * Significantly different from CTRL, p < 0.05. CTRL, control; AP, Arthrospira platensis; HP, Haematococcus pluvialis; PT, Phaeodactylum tricornutum; CV, Chlorella vulgaris; SCFA, short-chain fatty acid; BCFA, branched-chain fatty acid.