Table 5.
pH values, ammonia and short-chain fatty acids concentrations after 24 h of an in vitro incubation of canine fecal inoculum supplemented with microalgae 1.
Item | CTRL | AP | HP | PT | CV | Pooled SEM | Anova p-Value |
---|---|---|---|---|---|---|---|
pH | 5.84 | 5.84 | 5.81 | 5.95 | 5.81 | 0.01 | 0.004 |
Ammonia, mmol/L | 39.6 | 39.9 | 36.0 | 38.0 | 35.7 | 1.29 | 0.065 |
Straight-chain SCFA, mmol/L | |||||||
Acetate | 16.7 | 16.9 | 16.6 | 16.5 | 16.5 | 0.48 | 0.960 |
Propionate | 9.68 | 10.5 | 10.3 | 11.7 * | 10.7 | 0.28 | 0.001 |
Butyrate | 5.43 | 5.73 | 5.31 | 5.65 | 5.61 | 0.14 | 0.271 |
Total SCFA | 31.8 | 33.1 | 32.2 | 33.8 | 32.8 | 0.89 | 0.536 |
BCFA, mmol/L | |||||||
Isobutyrate | 0.60 | 0.64 | 0.60 | 0.64 | 0.62 | 0.02 | 0.289 |
Isovalerate | 0.92 | 0.95 | 0.90 | 1.01 * | 0.94 | 0.02 | 0.041 |
Total BCFA | 1.52 | 1.59 | 1.50 | 1.65 | 1.56 | 0.04 | 0.086 |
Individual SCFA proportions, % | |||||||
Acetate | 48.7 | 47.3 * | 47.9 * | 45.2 * | 46.7 * | 0.20 | <0.001 |
Propionate | 28.3 | 29.4 * | 29.7 * | 32.2 * | 30.2 * | 0.09 | <0.001 |
Butyrate | 15.9 | 16.0 | 15.4 * | 15.6 | 15.9 | 0.10 | 0.001 |
Isobutyrate | 1.76 | 1.80 | 1.74 | 1.76 | 1.75 | 0.01 | 0.041 |
Isovalerate | 2.68 | 2.66 | 2.59 | 2.78 | 2.65 | 0.05 | 0.254 |
1 Values are the means of five bottles per treatment. * Significantly different from CTRL, p < 0.05. CTRL, control; AP, Arthrospira platensis; HP, Haematococcus pluvialis; PT, Phaeodactylum tricornutum; CV, Chlorella vulgaris; SCFA, short-chain fatty acid; BCFA, branched-chain fatty acid.