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. 2022 Aug 1;10(8):1853. doi: 10.3390/biomedicines10081853

Table 1.

Chromatographic gradient used for analysis by LC-MS/MS. Phase A: 0.1% formic acid in water; phase B: methanol.

Time (min) Flow (mL/min) % A % B
0 0.8 40 60
1 0.8 40 60
10 0.8 0 100
12 0.8 0 100
12.10 0.8 40 60
17 0.8 40 60