Table 3.
Primers sequence employed for analysis of quantitative real-time PCR.
| Gene | Sequence | Accession No. |
|---|---|---|
| SOD | F-GACGTGACAACACAGGTTGC R-TACAGCCACCGTAACAGCAG |
XM_003449940.5 |
| CAT | F-TCAGCACAGAAGACACAGACA R-GACCATTCCTCCACTCCAGAT |
XM_031754288.1 |
| GSH-Px | F-CCAAGAGAACTGCAAGAACGA R-CAGGACACGTCATTCCTACAC |
NM_001279711.1 |
| IL-1β | F-TGCTGAGCACAGAATTCCAG R-GCTGTGGAGAAGAACCAAGC |
XM_019365841.2 |
| IL-6 | ||
| IL-8 | F-GCACTGCCGCTGCATTAAG R-GCAGTGGGAGTTGGGAAGAA |
XM_031747075.1 |
| IL-10 | F-CTGCTAGATCAGTCCGTCGAA R-GCAGAACCGTGTCCAGGTAA |
XM_013269189.3 |
| TNF-α | F-GAGGTCGGCGTGCCAAGA R-TGGTTTCCGTCCACAGCGT |
NM_001279533.1 |
| Caspase 1 | F: GCTGTCTGAGTAAGTGTATCAT R: CCAACACGTTAAAATGGATCTGA |
XM_019367762.2 |
| Atg5 | F-ATTGGCGTTTTGTTTGATCTT R-TTTGAGTGCATCCGCCTCTTT |
XM_019082404.1 |
| Atg12 | F-ACAGTACAGTCACTCGCTCA R-AAAACACTCGAAAAGCACACC |
XM_019125508.1 |
| LC3-II | F-GGAACAGCATCCAAGCAAGA R-TCAGAAATGGCGGTGGACA |
NM199604.1 |
| BCLN1 | F-TCTGTTTGATATCATGTCTGG R-TAATTCTGGCACTCATTTTCT |
XM_019068185.1 |
| mTOR | F-TGCGGAGTATGTGGAGTT R-CATCTCTTTGGTCTCTCTCTGG |
XM_019108641.1 |
| β-actine | F-TGGCATCACACCTTCTATAACGA R-TGGCAGGAGTGTTGAAGGTCT |
XM_003455949.2 |
GSH-Px: glutathione peroxidase, SOD: superoxide dismutase, CAT: catalase, IL: interleukin, TNFα: tumor necrosis factor α, LC3-II: Microtubule-associated proteins 1A/1B light chain, BCLN1: Beclin-1, mTOR: mechanistic target of rapamycin (mTOR).