Figure 10.

Comparisons of E_GABA derived from dual cell-attached and whole-cell recordings. (A) Summary plot of resting E_m and E_GABA obtained during CA and WC recordings of GABA-PSPs in CA1 pyramidal cells with [Cl⁻]_i = 4 mM and 30 mM in the WC pipette solution. Note that E_GABA values were calculated relative to E_m values in CA and WC, respectively. The difference in resting E_m values results in an apparent difference in E_GABA estimates in CA and WC recordings. (B) E_GABA values from the same dataset as in (A), recalculated as a function of E_m in WC both for WC and CA data. This approach minimizes the error caused by a difference in E_m values in CA and WC, and E_GABA values in CA and WC show no difference at both [Cl⁻]_i = 4 mM and 30 mM. (C) Corresponding p-value maps for all parameters shown in (A,B). The color of the cell at the row-column intersection shows the statistical significance (p-value) for the corresponding parameters. The dark red color corresponds to non-significant differences (p > 0.05), the other colors show different levels of significance of differences (p < 0.05) according to the color code (color bar on the right panel). (A–C) Pooled data from 8 pyramidal cells in the L5 somatosensory cortex of the mouse.