Figure 3.

Comparison of neuronal depolarization induced by elevated potassium during cell-attached and whole-cell recordings. (A) Scheme of recordings. (B) Example of the response to an increase in [K⁺]_o from 2.5 to 8 mM in the bath solution during dual WC and CA recordings in a L5 pyramidal neuron. The dashed lines indicate the steady-state E_m levels used to calculate E_m depolarization during [K⁺]_o elevation. The trace below shows a difference between the E_m values in CA and WC throughout the experiment. (C) Neuronal depolarization in response to high potassium in paired WC and CA recordings (left) and corresponding CA/WC transfer coefficient (right). Pooled data from 9 L5 pyramidal cells of mouse somatosensory cortex. n.s, non-significant.