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. 2022 Aug 11;15:979479. doi: 10.3389/fnmol.2022.979479

Figure 7.

Figure 7

Action potentials in cell-attached and whole-cell recordings. (A) Scheme of recordings. APs are evoked by suprathreshold depolarizing current steps applied to the WC electrode. (B) Example of a response to a suprathreshold current step applied to the WC electrode during dual WC and CA recordings from a L5 pyramidal neuron. The ice-blue and blue traces show WC responses before (WC) and after (WC bridge -) bridge subtraction, respectively. Note that the bridge current is absent in the CA trace (inset). (C) APs in the CA and WC recordings with common voltage scale (top panel) and normalized to peak amplitude (bottom panel), dashed line is placed at half-amplitude. The resting membrane potential values were subtracted. (D) AP parameters in paired WC and CA recordings: AP amplitude (left), half-duration (middle), and time from onset to peak (right). The insets show in green the values of corresponding CA/WC transfer coefficients. Pooled data from 12 pyramidal cells in the L5 somatosensory cortex of the mouse. ***P < 0.001.