Figure 9.

Oral larazotide treatment normalizes increased gut permeability induced by RG strain neonatal colonization. (A) Plasma zonulin levels in 14-week-old mice after neonatal colonization, were significantly elevated in S107-48 RG strain mice compared to non-colonized controls (n = 5 to 12 mice). For each group mean+/-SD. In littermates from RG colonized SPF breeding pairs, individual mice were retested with results shown (before), then after a ten-day treatment with larazotide peptide in the water supply and 48 hr rest, gut permeability was then retested (after), with plasma FD4 levels shown for; (B) RG2 strain colonized mice, (C) S107-48 RG Lupus strain colonized mice, (D) S47-18 RG Lupus strain colonized mice, with comparison to (E) levels detected in non-colonized control mice. For the bottom panels, significance was tested by paired t test. p<0.05 was considered statistically significant.