Table 1.
TLR Pathway |
Primer Sequence (5′ to 3′) | Gen-Bank Accession Number |
---|---|---|
Nrf2 | 5′ CAT TTGTAGATGACCATGAGTCGC 3′ (sense) 5′ ATCAGGGGTGGTGAAGACTG ′ (antisense) |
NM_031789.2 |
HO-1 | 5′ GTGCACATCGTGCAGAGAA 3′ (sense) 5′ GTGCACATCCGTGCAGAGAA3′ ′ (antisense) |
NM_012 580.2 |
NQO1 | 5′-AGGATGGGAGGTACTCGATC -3′ (sense) 5′-AGGCGTCCTTCCTTATATGCTA -3′ ′ (antisense) |
NM_008706.5 |
TLR2 | 5′-ATGAACACTAAGACATACCTGGAG-3′ (sense) 5′-CAAGACAGAAACAGGGTGGAG-3′ (antisense) |
NM_198769 |
TLR4 | 5′-CATGACATCCCTTATTCAACCAAG-3′ (sense), 5′-GCCATGCCTTGTCTTCAATTG-3′ (antisense) |
NM_019178 |
MyD88 | 5′-GAGATCCGCGAGTTTGAGAC-3′ (sense) 5′-CTGTTTCTGCTGGTTGCGTA-3′ (antisense) |
NM_198130.2 |
NFκB | 5′-ATCATCAACATGAGAAACGATCTGTA-3′ (sense) 5′-CAGCGGTCCAGAAGACTCAG-3′ (antisense) |
L26267.1 |
Bax | 5′-GTGGTTGCCCTCTTCTACTTTG-3′ (sense) 5′-CAAAAGATGGTCACTGTCTGC-3′ (antisense) |
NM_017059.2 |
Bcl-2 | 5′-CCGGGAGATCGTGATGAAGT-3′ (sense) 5′-ATCCCAGCC TCCGTTATCCT-3′ (antisense) |
NM_016993.1 |
β-Actin | 5′-TGCTATGTT GCCCTAGACTTCG-3′ (sense) 5′-GTTGGCATAGAG GTCTTTACGG-3′ (antisense) |
NM_031144 |