FIG. 3.

CBP-ZmaR acetyltransferase reactions. Acetyltransferase reaction mixtures contained CBP-ZmaR or protein elution buffer, zwittermicin A, and [1-¹⁴C]acetyl-CoA. Samples were separated by TLC on silica 60 gel plates with n-butanol–acetic acid–water (2:1:1) as solvent. TLC plate was visualized on a PhosphorImager. Lanes: 1, free acetyl-CoA; 2, acetyltransferase reaction with zwittermicin A omitted; 3, acetyltransferase reaction with CBP-ZmaR omitted; 4, complete acetyltransferase reaction; 5, complete acetyltransferase reaction with one-tenth the amount of CBP-ZmaR. Acetylated zwittermicin A was identified by comigration with authentic HPLC-purified acetylated zwittermicin A (not shown).