TABLE 3.
Inactivation of zwittermicin A by CBP-ZmaR
| Treatmenta | Zone size (mm) |
|---|---|
| Elution buffer + zwittermicin A + acetyl-CoA | 3.0 |
| CBP-ZmaR + zwittermicin A + acetyl-CoA | 0.0 |
| Elution buffer + zwittermicin A + acetyl phosphate | 2.5 |
| CBP-ZmaR + zwittermicin A + acetyl phosphate | 2.5 |
| Elution buffer + zwittermicin A + ATP | 3.0 |
| CBP-ZmaR + zwittermicin A + ATP | 3.0 |
a
Treatments contained 4 μg of zwittermicin A, 100 ng of CBP-ZmaR, and either acetyl-CoA (5 mM), acetyl phosphate (4 mM), or ATP (4 mM). Reactions were incubated at 28°C for 16 h. Protein elution buffer (50 mM Tris-Cl [pH 8.0], 10 mM β-ME, 2 mM EGTA, 0.05% Triton X-100, 20% glycerol) was included in place of CBP-ZmaR as a mock treatment. Reaction mixtures were spotted onto 10MH8.1 agar against a lawn of E. coli and scored for zones of inhibition after 24 h. Data reported are representative of three experiments.