qPCR reaction master mix
| Reagent | Final concentration | Volume |
|---|---|---|
| 2 × GoTaq master mix | 1× | 5 μL |
| H2O | N/A | 2.95 μL |
| Primer set | 0.25 μM | 0.025 × 2 μL |
| cDNA | N/A | 2 μL |
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