REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Flag, M2, 1:2000	Sigma-Aldrich	Cat#F1804, RRID: AB_262044
Bacterial and virus strains
LV-Rep/Ref	(Abe and Abe, 2022)	N/A
Chemicals, peptides, and recombinant proteins
2-Mercaptoethanol	Wako	Cat#133-14571
2-Propanol	Wako	Cat#163-04846
8-Hydroxyquinoline	Nacalai Tesque	Cat#18912-52
Butorphanol tartrate	Meiji Seika Pharma	Vetorphale
Calcium chloride	Wako	Cat#030-25045
Chloroform	Wako	Cat#035-02616
D(+)-Glucose	Wako	Cat#046-31175
4′,6-Diamidino-2-phenylindole (DAPI)	Dojindo Molecular Technologies	Cat#D523
Diethylpyrocarbonate	Nacalai Tesque	Cat#12311-86
Dulbecco’s Modified Eagle Medium (DMEM)	Wako	Cat#044-29765
Ethanol	Nacalai Tesque	Cat#14710-25
Fatal bovine serum (FBS)	Biowest	Cat#S1530
FCF Fast Green	Nacalai Tesque	Cat#15939-54
GoTaq qPCR Master Mix	Promega	Cat# A6002
Guanidinium thiocyanate	Nacalai Tesque	Cat#06287-45
HEPES	Nacalai Tesque	Cat#02443-05
Magnesium sulfate	Wako	Cat#034-00925
Medetomidine hydrochloride	All Japan pharma	Domitol
Midazolam	Astellas Pharma	Dormicum
N-Lauroylsarcosine sodium salt solution	Nacalai Tesque	Cat#20135-14
Opti-MEM I	Thermo Fisher Scientific	Cat# 31985070
PBS	N/A	N/A
PEI-MAX	Polysciences	Cat# 24765
Penicillin / Streptomycin	Wako	Cat#168-23191
Phenol	Nacalai Tesque	Cat#26728-45
Polyethylene glycol 6,000	Nacalai Tesque	Cat#28254-85
Potassium chloride	Wako	Cat#160-03555
ReverTra Ace qPCR RT Master Mix with gDNA remover	Toyobo	Cat#FSQ-301
Sodium acetate	Wako	Cat#199-01085
Sodium chloride	Wako	Cat#198-01675
Sucrose	Wako	Cat#196-00015
Sodium citrate	Wako	Cat#198-01795
Experimental models: Organisms/strains
Mouse: Slc:ICR (Embryonic day 15, 16-day-old, and 8-week-old male mice)	SLC Japan	N/A
Oligonucleotides
RT-primer	(Abe and Abe, 2022)	See Primer List in Table 1.
qPCR-primer	(Abe and Abe, 2022)	See Primer List in Table 2.
Recombinant DNA
pLV-Rep/Ref	(Abe and Abe, 2022) Addgene	#182054, #182055, #182056, #182057, #182058, #182059
pRSV-Rev	Addgene	Cat#12253
pMD2.G	Addgene	Cat#12259
pMDLg/pRRE	Addgene	Cat#12251
Other
0.45 μm pore filter	Nalgene	Cat#121-0045
Artery forceps	Frigz	Cat#E560-845
Beads mill homogenizer	Bio-Medical Science	Cat#45-1291
Dissecting scissors	Frigz	Cat#D340-476
Extension tube	Terumo	Cat#7-4621-06
Fine glass capillaries	Harvard Apparatus	Cat#30-0038
Heating pad	Vivaria	Cat#LP-817
LightCycler 480 Multi-well Plate 384, white	Roche Diagnostics	Cat#04729749001
LightCycler 480 Sealing Foil	Roche Diagnostics	Cat#04729757001
LightCycler 480 System II	Roche Diagnostics	Cat#05015243001
Low adsorption microtubes	Treff	Cat#96.07246.9.01
Membrane filter	ADVANTEC	Cat# 63-1237-28
Micropipette beveler	Narishige	Cat#EG-45
Micropipette puller	Narishige	Cat#PC-100
Screw cap tube, 2.0 mL	Simport	Cat#T330-7
Stainless steel beads, 5 mm diameter	AS ONE	Cat#2-9244-05
Surgical suture	Bear Medic	Cat#61-9190-12
Syringe	Terumo	Cat#1-4908-01
Thermal cycler	Bio-Rad	Cat#1861096
Tissue forceps	Frigz	Cat#A049-0751