RT-PCR conditions
| Steps | Temperature | Time |
|---|---|---|
| Reverse transcription reaction | 37°C | 15 min |
| Reverse transcription reaction | 42°C | 5 min |
| Reverse transcription reaction | 50°C | 5 min |
| Enzyme inactivation reaction | 95°C | 5 min |
| Hold | 4°C | Forever |
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