Figure 3.

Fluorescent images show bead sensor arrangement and captured analyte, with variation in signal intensity at various concentrations (A). Image analysis was performed by measuring 95% ROI on sensor beads on a dark subtracted image captured during last stage of assay (B). The SARS-CoV-2 antibody concentration was assessed on the McDevitt lab point-of-care quantitative immunity scoring assay tool, and the dose curve here depicts a dynamic range between 10⁰ and 10⁵ (with potential to capture titers up to ~10⁶); all values depicted in the curve are represented by mean and error bars—standard deviation (C). Subsequently, signal-to-noise ratio (SNR) was calculated to indicate assay robustness and functionality (D).