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Time course of chitinase production by Alteromonas sp. strain O-7 cultured in media containing various carbon sources. Aliquots were taken at the indicated times and centrifuged; chitinase activity was measured with PNP-(GlcNAc)2 as a substrate. Symbols: ■, 0.1% chitin; □, 1% β-(1→6)-(GlcNAc)2; ▴, 0.1% β-(1→6)-(GlcNAc)2; ▵, 0.1% glucose; ▾, 0.1% (GlcNAc)2; ●, 0.1% GlcNAc; ○, 0.1% (GlcNAc)3; ⧫, no addition. Data represent the means of three independent experiments.
