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Western blot analysis of TasA during growth in 2×YT. Extracts of cells grown in 2×YT were prepared at the indicated times before and after the onset of stationary phase (T0). After fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransfer to polyvinylidene difluoride membranes, TasA was detected with an anti-TasA antibody. Molecular mass is indicated in kilodaltons on the left.
