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. 1999 Sep;181(17):5476–5481. doi: 10.1128/jb.181.17.5476-5481.1999

FIG. 4.

FIG. 4

β-galactosidase activity in cells bearing yqxM-, sipW-, or tasA-lacZ. All strains harbor either sipW-lacZ (AGS301), yqxM-lacZ (AGS307), no fusion (PY79 and JH642), or tasA-lacZ (all remaining strains). In each plot, the onset of stationary phase (time zero) is indicated by an arrow. (A) Fusions to tasA, sipW, or yqxM and a tasA-lacZ fusion in which the 351 bp upstream of yqxM have been deleted. Strains tested: AGS301 (yqxM-lacZ) (▵), AGS307 (sipW-lacZ) (○), AGS303 (tasA-lacZ) (□), AGS340 (yqxM upstream sequences deleted, tasA-lacZ) (✻), and PY79 (⧫). (B) tasA-lacZ fusions retaining various regions upstream of yqxM. Strains tested: AGS303 (retaining 351 bp) (□), AGS376 (281 bp) (▵), AGS377 (264 bp) (○), AGS378 (233 bp) (✻), AGS379 (114 bp) (●), AGS380 (40 bp) (+), AGS381 (30 bp) (■), and PY79 (⧫). (C) Effects of spo0A and spo0H. Strains tested: AGS303 (wild type) (□), AGS305 (spo0HΔHindIII-EcoRI::cat::Specr) (▵), AGS313 (spo0AΔ::Ermr) (○), and PY79 (⧫). (D) Effects of abrB. Strains tested: AGS303 (wild type) (□), AGS351 (ΔabrB::Tn917) (▵), AGS352 (ΔabrB::cat spo0HΔHindIII-EcoRI::cat::Specr) (○), AGS392 (abrB::Tn917::pSpc101 spo0AΔ::Ermr) (✻), and PY79 (⧫). (E) Effect of deletion of the ςH-driven promoter of spo0A. Strains tested: AGS387 (wild type) (□), AGS367 (spo0AΔPs) (▵), and JH642 (⧫). (F) Effects of alleles of spo0A deficient in ςA-dependent gene expression. Strains tested: AGS303 (wild type) (□), AGS361 (spo0A::pSPC101) (▵), AGS362 (spo0A::pSPC101-S233P) (×), AGS363 (spo0A::pSPC101-G227R) (✻), AGS364 (spo0A::pSPC101-F236S) (●), AGS365 (spo0A::pSPC101-V240A) (+), AGS366 (spo0A::pSPC101-V240G K265R) (■), and PY79 (⧫). Units of β-galactosidase activity are defined in Materials and Methods. Scales along the y axis vary from panel to panel; in particular, the scale in panel D is compressed.