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. 2022 Aug 18;10(8):2009. doi: 10.3390/biomedicines10082009

Figure 7.

Figure 7

Effect of fraction III (6 µg/mL) on α-synuclein accumulation (A,D) and mitochondrial fission (B,C), evaluated by anti-α-synuclein antibody and Mitotracker Red CMXRos, respectively, via confocal microscopy on SH-SY5Y cells treated with 6-OHDA (50 µM). Quantitative analysis was conducted on ImageJ, version 1.47. Data are expressed as mitochondrial length (µm) (C) or mean fluorescence intensity (D). Scale bars: 10 μm. N ≥ 30. Results are showed as mean ± standard deviation (SD) from three independent experiments. ### denotes p < 0.001 vs. Ctrl; **, *** denote p < 0.01 and p < 0.001 vs. 6-OHDA.