Figure 4.
HSP70 Inhibition increases ROS production in the aorta of middle-aged animals but does not affect the levels of nitric oxide. (A) The fluorescent probes H2DCFDA, DHE, and DAF-FM were used to measure the levels of vascular oxidative stress and nitric oxide in isolated aortic rings from middle-aged rats incubated with vehicle or VER155008 (10−5 mol/L) for 6 h. Fluorescent intensity for H2DCFDA (B), DHE (C), and DAF-FM (D) are displayed as arbitrary units. Scale bar: 50 µm. Data are expressed as mean ± SEM. (n = 5–6), * p < 0.05 and ns. p > 0.05 vs. middle-aged.