Table 3.

Differentially expressed genes (DEGs) up and downregulated involved in the “Regulation of fatty acid metabolic process”, “Regulation of lipid metabolic process” and “Regulation of carbohydrate metabolic process”.

Biological Process		Upregulated DEGs	Fold Change	Downregulated DEGs	Fold Change
Fatty acid metabolic process	Positive regulation	CPT1A	0.62	PPARG	−1.20
	Negative regulation			INSIG1	−2.00
				PIBF1	−0.46
				SIRT4	−3.97
Lipid metabolic process	Positive regulation	BMP6	1.71	FGF2	−0.81
		CPT1A	0.62	PPARG	−1.20
		PDGFRB	1.04	SIRT4	−3.97
		PTK2B	1.27	SORBS1	−1.36
	Negative regulation	LPCAT1	0.73	HCAR1	−2.38
				INSIG1	−2.00
				PDE3B	−1.53
				PIBF1	−0.46
				SIRT4	−3.97
				SOD1	−1.21
Carbohydrate metabolic process	Positive regulation			ARPP19	−1.07
				IGF1	−0.95
				PRKAA2	−5.69
				PRXL2C	−0.95
				SNCA	−0.81
				SORBS1	−1.36
	Negative regulation	HDAC4	1.11	EP300	−0.19
		MST1	2.91

DEGs involved in the “Regulation of fatty acid metabolic process”, “Regulation of lipid metabolic process” and “Regulation of carbohydrate metabolic process” with fold change associated. Fold change was calculated as log₂ (AT-OB/AT-Ctrl). In this line, the upregulated genes were more expressed in AT-OB while downregulated genes were more expressed in AT-Ctrl. The DEG is specified if it is involved in positive or negative regulation. All the values are rounded to the second decimal digit.