Figure 4.

FAM107A overexpression inhibited the proliferation of PCa cells in vitro. (A) The Western blotting analysis confirmed the effect of FAM107A overexpression. The unit of molecular weight is kDa. (B) Comparison of the proliferative capacity of the two groups of cells, detected using the CCK-8 kit, with OD values measured every 24 h. (C,D) Comparison of the colony-forming ability of the two groups of cells. Colony formation was quantified 14 days after colony formation using ImageJ software. (E) Immunofluorescence was used to detect Ki67 expression in the cells at a magnification of 400×. Similar results were obtained, and data are expressed as the mean ± SD of at least three experiments. *** for p < 0.001, and ** for p < 0.01.