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View full-text article in PMC

. 2022 Aug 13;14(16):3915. doi: 10.3390/cancers14163915

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Overexpression of FAM107A inhibited PCa cell migration in vitro as well as invasion. (A,B) The migration ability of cells was assessed using a wound healing assay. The healing area of wounds was quantified by ImageJ software 24 h after wound formation. (C,D) Cell invasiveness was assessed using a Transwell invasion assay. The number of invaded PCa cells was quantified with ImageJ software. (E) Inhibition of EMT by overexpressed FAM107 was verified by Western blotting, and molecular weights are in kDa. (F) Immunofluorescence assays were performed to detect E-cad and Vimentin expression in cells at a magnification of 400×. Data are expressed as the mean ± SD of at least three experiments. *** for p < 0.001, and ** for p < 0.01.